Zob Ahan was defeated by Saipa 2-0. Karim Ansarifard and Amin Manouchehri scored the goals in the 17th and 57th minutes.
Zob Ahan remains on top of the chart with a six-point lead over Sepahan who was held to a goalless draw by Shahin in Bushehr.
Tehran-based Persepolis beat Sanat Naft of Abadan 2-0 at the Azadi Stadium. Maziar Zare gave Persepolis a lead from the penalty spot just before the break, and Hadi Norouzi scored the second goal for the host in the 52nd minute.